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It is worth noting that in the normal liver microenvironment, Kupffer cells possess excellent clearance capabilities

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Furthermore, it may alter the transcript levels of genes involved in oogenesis, folliculogenesis, and embryonic development 12 and impair nuclear and cytoplasmic maturation events such as the translocation of cortical granules and cytoskeletal rearrangement 13 , which can eventually lead to apoptosis 14

Telomere length in cumulus cells is positively correlated with oocyte and embryo quality (Keefe et al., 2007)

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Following the blocking step, the plates were washed four times with the washing reagent and incubated with 100 l of the standards and samples for 2 h at room temperature with shaking