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Heat stress accelerates oxidative damage in oocytes, as demonstrated in bovine studies where gradual exposure to elevated temperatures (38,540,5 C) induced oxidative stress and reduced expression of interferon tau (IFNT), a cytokine essential for embryo-maternal communication [25]

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The dithio-binitrobenzoic acid method was used to measure the GPx activity, and the absorbance was read at 412 nm

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