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Brief handling at room temperature is usually unavoidable, but storage at room temperature is not recommended

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These approaches target the issues that create the shelf in the first place

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Cells were spun at 4C for 20 min to pellet, and resuspended in lysis buffer (50 mM NaH2PO4, 300 mM NaCl, 10 mM imidazole, pH = 8)

These are the questions that will move the science from compelling hypothesis to established fact