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For the OGD phase, cells were washed twice with PBS and then incubated in glucose-free DMEM (Gibco, Thermo Fisher Scientific, Waltham, MA, United States) under hypoxic conditions (1% O 2 , 94% N 2 , 5% CO 2 ) in a hypoxia chamber (BioSpherix, Parish, NY, United States) for 8 h

Many biohackers love TMG because it targets the root cause behind symptoms

Urinary citrate and renal stone disease: the preventive role of alkali citrate treatment

However, dedicated immunogenicity data are sparse

Carbohydr Polym 261(12):117863

The resulting modified proteins were then digested with 40 L sequencing grade trypsin solution (0.05 g/l) for 16 h at 37 C