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Culture media from 24-hour incubated cells were collected, stored at -80 C, and analyzed for urea concentration using LC/MS/MS API3000, normalized to total cell protein

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GHK-Cu is often studied with other regenerative and anti-aging compounds

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Simone Tan Institution: Singapore Institute for Neuroregeneration Result: Peptide-based circadian entrainers (like vasoactive intestinal peptide) combined with autologous stem cells post-plasmapheresis led to substantial reduction in insomnia severity index scores and improved subjective sleep quality in clinical pilot trials