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This enzyme catalyses the transfer of the -glutamyl moiety of GSH to another amino acid to produce a -Glu-AA and cysteinyl-glycine

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As a consequence, the main mitochondrial proteome (~1500 proteins) is encoded by the nucleus, translated in the cytosol and imported into the mitochondria through specific translocator complexes (TIM and TOM) of the inner mitochondrial membrane (IMM) and outer mitochondrial membrane (OMM), respectively