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Differences in stimulation regimens, progesterone exposure timing, sampling windows, culture media formulation, incubator conditions, and operator-dependent handling can generate centre-specific signatures that mimic biology in single-site studies but weaken or invert in external cohorts [461, 462, 482, 483]

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Brain glucose levels are elevated in chronically hyperglycemic diabetic rats: no evidence for protective adaptation by the blood brain barrier

After 24 weeks of treatment, blood samples are collected after the eyeballs of the mice are extracted for serum preparation by centrifugation at 2000g for 10 min at 4C