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Cells were mixed with transfection reagents and plated at 13,000 cells per well into 96-well Greiner CELLSTAR white-walled plates (Sigma-Aldrich), and assays were performed 48 h later

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However, some homocysteine is used in the Transulphuration pathway to make other useful substances like glutathione.

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Development of an enzyme immunoassay for delta sleep-inducing peptide (DSIP) and its use in the determination of the metabolic clearance rate of DSIP administered to dogs