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For separate vials, calculate each vial separately

k , Proteomic profiles for phosphorylation-dependent labeling by P2 (left) and glycosylation-dependent labeling by ( S )-P6 (middle), alongside a docked structure (AF-Q8N8Q9-F1) showing the distinct binding sites (right)

Plant Physiol Biochem 207:108393

This dead volume, typically 0.02 to 0.07 mL depending on the syringe, means you lose a small amount of solution with each draw

And the pineal cells themselves degenerate

Elimova, E, Wang X, Qiao W, Sudo K, Wadhwa R, Shiozaki H, Shimodaira Y, Planjery V, Charalampakis N, Lee J, Weston B, Bhutani M, Komaki R, Rice D, Swisher S , Blum M, Rogers J, Skinner H, Maru D, Hofstetter W