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Subsequently, the cell monolayer was scratched using a 200 L pipette and then washed twice with PBS

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Importantly, serum B12 levels can be misleadingly normal or even elevated in chronic liver disease due to increased levels of binding proteins (such as haptocorrin) and reduced clearance, meaning that total B12 measurements may not accurately reflect tissue B12 status or functional deficiency

Technical Challenges Oxidative Degradation: The peptide degraded within 14 days under standard accelerated aging conditions (40C)