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For HFD feeding, the mice were maintained on a HFD (D12492) (60.0 % kcal from fat) (Research Diets Inc., New Brunswick, NJ, USA) starting at 8 weeks of age unless stated otherwise

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The peptide influences both endothelial nitric oxide synthase (eNOS), which produces the beneficial, vasodilating form of nitric oxide, and inducible nitric oxide synthase (iNOS), which can produce excessive amounts that contribute to inflammation and tissue damage