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Nanoparticles were diluted in phenol red-free DMEM/HAMs F-12 medium (Gibco, Life Technologies, Carlsbad, CA, USA) supplemented with 5% FBS at a final concentration of 100 g/ml and added to the donor compartment (0.2 ml)

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and P50AR070588, R01AR065963, R01AR056016, U01AR067173 and R21AR061881 to A.M.C.) and the National Alopecia Areata Foundation (L.E.K., A.M.C

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