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It is characterized by the presence of an extra copy of chromosome 21 and can result from various genetic abnormalities such as maternal non-disjunction, mosaic karyotype, Robertsonian translocation, or partial duplication of the critical region of chromosome 21(Hoeffer et al

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After 19 h incubation in a humidified CO 2 /air incubator, the ring-enriched fraction was separated on and collected from a discontinuous 40/80/90% Percoll gradient, containing mannitol (6% wt/vol)