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Knocking down YAP effectively prevented PQQ-mediated reductions in ROS levels in the PE + PQQ + si-YAP treatment group (Figure 5F)

Gs recruitment assay G protein recruitment to CTR or individual AMYR subtypes was measured in COS-7 cells (ATCC, CRL-1651) transfected with c-myc-hCTRaleu-Rluc8:Gs-Venus:G1:G2, c-myc-hCTRaleu:RAMP1 Nluc:Gs-Venus:G1:G2, c-myc-hCTRaleu:RAMP2-Nluc:Gs-Venus:G1:G2 or c-myc-hCTRaleu:RAMP3-Nluc:Gs-Venus:G1:G2 at a ratio of 2:0.1:1:1:1, in a total of 51 ng DNA per well using PEI at a ratio of 1:6 of DNA to PEI

Cerqueira et al., 2020)

H.PastanI.et al (1981)

As evident from Figures 2A,B, the colon length of mice with DSS-induced colitis was notably shorter than that of the Control group