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All cell lines tested negative for mycoplasma contamination using a MycoAlert Mycoplasma Detection kit (Lonza, LT07-418)
3.3.3 Receptors mediated mitophagy activators Panax notoginseng saponins (PNS), the primary ingredient from Panax notoginseng, exhibits extensive therapeutic effects in cardiovascular diseases (Yang et al., 2016) and diabetes (Uzayisenga et al., 2014)
however, once the balance between ROS production and clearance is disrupted, excessive ROS generation can lead to heightened oxidative stress (17)
10.1038/nm0898-901 92
Yan SD, Schmidt AM, Anderson GM, Zhang J, Brett J, Zou YS, Pinsky D, Stern D: Enhanced cellular oxidant stress by the interaction of advanced glycation end products with their receptors/binding proteins
The kit utilizes a colorimetric substrate that reacts with the free thiol group on GSH to yield a highly colored product
