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The k syst values were obtained, at various loading rates, from the slope of the retraction curve immediately prior to the unbinding event

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Our treatment options aim to give you more energy so you can work and enjoy your free time without pain and exhaustion

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the membranes were then washed several times with water and TBS (PBS, 0.05% Tween-20), a 1:5000 dilution of horseradish-peroxidase conjugated goat anti-rabbit antiserum was added, and the membranes further incubated for one hour at room temperature

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