Cystine uptake assay The cystine uptake assay was conducted with BioTracker Cystine-FITC Live Cell Dye (#SCT047, Sigma)
It's a good idea to ask your doctor about having your B 12 level checked if you are a strict vegetarian or have had weight-loss surgery or have a condition that interferes with the absorption of food
For validation of the candidate genes, the individual lentivirial plasmid lentiCRISPR v2 containing the top two sgRNAs for each candidate gene, one sgRNA targeting GGCX (positive control), and one non-targeting sgRNA (negative control) were co-transfected with the helper plasmids in HEK293T cells as described above using Xfect transfection reagent
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FIGURE 2 atgrxs17 Plants Accumulate More Iron in Seeds To determine whether disruption of AtGRXS17 affects ferric chelate reductase, both wild type control and mutant seedlings were grown on iron sufficient or deficient medium for 3 days
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