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doi:10.1016/j.amjoto.2020.102640

In vitro hypoxia and nutrient deprivation model Cells were cultured to approximately 80% confluence, then the medium was replaced with EBSS containing 200 M cobalt chloride for the indicated times [55]

2.4 BMD measurement, osteopenia and osteoporosis diagnoses Lumbar spine (L1L4) BMD and femoral neck BMD were measured by the Hologic QDR-4500A fan-beam densitometers (Hologic, Bedford, MA) and analyzed by Hologic APEX software (Version 4.0, Hologic, Bedford, MA)

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