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The animals were group-housed until one week before treatment initiation, after which they were given daily subcutaneous injections of vehicle or Cagrilintide at doses of 0.3, 3, or 30 nmol/kg for 21 days

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For drug treatment, BAY-876 (MedChemExpress, HY100017

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The cells were grown in a defined medium containing DMEM/HAM F12, supplemented with 33 mM glucose, 2 mM glutamine, 100 U/ml penicillin, 100 g/ml streptomycin, 13 mM sodium bicarbonate, 5.5 g/ml transferrin, 10 g/ml insulin, 1 pM -estradiol, 3 nM triiodothyronine, 20 nM progesterone, 5 ng/ml sodium selenite, and 100 M putrescine

(Oxf.) 73 , 535539 (2010)