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Each sample (8 l) was injected onto an Agilent Poroshell 120 EC-C18 (100 2.1 mm, 2.7 um) in a Shimadzu Nexera UHPLC system at a flowrate of 0.2 ml/min using a gradient elution created between buffer A (10 mM tributylamine aqueous solution adjusted pH to 4.95 with 15 mM acetic acid) and buffer B of methanol, from 10% to 40% (buffer B) in 30 min at room temperature
developed a PDT-based system using MOF PCN- 224 as a carrier for chloroquine (CQ), an autophagy inhibitor
By using advanced laboratory methods, the researchers examined how THI reacts with these biomolecules under conditions similar to those in the human body
BPC-157 stands for 'Body Protection Compound 157.' It's a synthetic peptide, which is just a short chain of amino acids
Any haziness indicates peptide aggregation or contamination