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Autophagy induction by SIRT6 through attenuation of insulin-like growth factor signaling is involved in the regulation of human bronchial epithelial cell senescence

Murakami T, Nishiyama T, Shirotani T, Shinohara Y, Kan M, Ishii K, Kanai F, Nakazuru S, Ebina Y

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Wild-type IR and IGF1R as well as chimeric receptors IR/IGF1R (IR-ECD (aa 1919) fused to IGF1R transmembrane and ICD (aa 9081,339), numbers excluding signal peptide) and IGF1R/IR (IGF1R-ECD (aa 1907) fused to IR transmembrane and ICD (aa 9201345)) were subcloned into the pBabe-hyrgromycin vector