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Quantitative measurement of NNMT reaction product 1-MNA in cultured cells Cellular 1-MNA concentrations were determined using an ultra-sensitive high-resolution AB Sciex 6500 Q-trap mass spectrometer coupled to an Agilent 1260 ultra-high pressure liquid chromatography (LC/MS/MS) system

Additionally, some individuals may have genetic variations in AMPK or NNMT expression that reduce responsiveness to these compounds, though this is less common

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