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Glutathione helps protect cells from free radical damage by acting as an antioxidant

For shRNA experiments, sh SLC7A11 was induced with doxycycline chow (600 mg kg 1 , Specialty Feeds, Australia) and water (2 mg ml 1 , Sigma-Aldrich) available ad libitum

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Most chronic illnesses are associated with cellular dysfunction within the body

A cloudy solution indicates a problem, most likely contamination or an issue with the solvent used