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PVDF membranes were blocked with 5% skim milk at room temperature for 2 h and then incubated with primary antibodies at 4 C overnight, followed by incubation with secondary horseradish peroxidase-conjugated antibodies (1:1000, Sungene Biotech, China) for 1 h at room temperature

After centrifugation, samples were measured by liquid chromatography coupled to tandem mass spectrometry (LC-MS/MS)

Serum Homocysteine Showed Potential Association with Cognition and Abnormal Gut Microbiome in Major Depressive Disorder. World Journal of Psychiatry, vol

Gut microbiota-derived D-serine protects against acute kidney injury