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Note that most animal studies use doses between 10 ng/kg and 10 g/kg, which corresponds to very small volumes in murine practice and imposes intermediate dilutions

10.1016/0013-9351(82)90139-6 34 MoscatelliN.LunettiP.BracciaC.ArmirottiA.PisanelloF.De VittorioM.et al (2019)

There are also microbial contamination concerns with refillable vessels over time

Kashani, L., et al

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