Amino acid and protein modification by oxygen and nitrogen species
For cell immunofluorescence staining, a similar procedure was performed, except the cell samples were fixed with 4% paraformaldehyde (PFA) (Beyotime, #P0099) for 10 min and permeabilized with 0.5% Triton X-100 (Beyotime, #P0096) for 10 min at room temperature
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In all experiments, age-matched mice were used (unless otherwise stated), WT littermates served as controls, and treatment was randomly assigned to control for cage effect
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Dwyer, N
