The rest period follows the completion of the full protocol, not each individual cycle
Particularly, the 4-OF cells stopped growing towards the end of the experiment at passage 18 (Fig
17 : 25962606 Ichijo H, Nishida E, Irie K, ten Dijke P, Saitoh M, Moriguchi T, Takagi M, Matsumoto K, Miyazono K and Gotoh Y (1997) Induction of apoptosis by ASK1, a mammalian MAPKKK that activates SAPK/JNK and p38 signaling pathways
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