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Cells were plated (2 10 7 cells per 150 mm plate) and grown for 24 h before transfection with Polyethylenimine (PEI, Sigma) according to the manufacturer's instructions

Forty-eight hours after transfection, cells were incubated in ice cold media and slides were placed on ice for 15 min

To improve the physicochemical and pharmacokinetic properties of YHV984 for specific Hv1 inhibition in the brain, the multifunctional nanoparticles consisting of a T7-targeting peptide and lipid nanoparticles (LNP) were developed to deliver YHV984 (T7-LNP@YHV984)

This peptide, made of only 15 linked amino acids, was originally derived from stomach tissue

Soto-Gamez, A