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A quick shot should never replace a thorough explanation

The expression of target genes was analyzed via quantitative real-time (RT) PCR with a QuantaStudio 5 qPCR machine (Applied Biosystems, Thermo Fisher Scientific)

Biochemically, silica stimulates rice to rapidly produce defense compounds such as phenolics, flavonoids, lignin, callose, and plant toxins, while also increasing the activity of defense enzymes such as peroxidase, phenylalanine ammonia-lyase, polyphenol oxidase, lipoxygenase, and -1,3-glucanase [27]

For our simplified model, we used the velocity expression for glutathione reductase reported in both of the aforementioned models in literature

Protein structural information derived from NMR chemical shift with the neural network program TALOS-N

J Agric Food Chem 54:72427250