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Immunoblot assays Tissue samples were crushed on dry ice to obtain homogenous aliquots and then lysed in RIPA buffer (Thermo Scientific #89900) containing Halt protease & phosphatase inhibitor (Thermo Scientific #1861280)

Similar approaches are also in preparation for other AD targets, such as TREM2 using a bi-specific anti-TREM2 antibody fused with scFv targeting TfR

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CL Answer Supplements to Take or Avoid When Using a GLP-1 Drug Certain supplements may be helpful when taking a GLP-1 agonist, but others should be used with caution or avoided

Heldner et al