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Retroviral infection was carried out transfecting HEK293T cells with pBS-CMV-gagpol (Addgene, #35614) and pCAG-VSVG (Addgene, #35616) vectors in addition to pLPCX cyto Grx1-roGFP2 (Addgene, #64975) and pLPCX myto Grx1-roGFP2 (Addgene, #64977)
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The 6th Edition of the World Health Organization (WHO) manual for human semen analysis classifies seminal OS testing as a research tool, emphasizing the lack of robust evidence to confirm its clinical utility (208)
Therefore, a variety of experimental studies and calculations consistently suggest a short half life in the 23 hour range
This work was supported by Genentech as part of the Alliance for Therapies in Neuroscience and the National Cancer Institute (NIH, R01 CA285366) (J.H.H.)
KashiwayaYPawloskyRMarkisWKingMTBergmanCSrivastavaSet al
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