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In the end point mode, mixing the sample or standard with the supplied Detection Reagent and GSH yields a fluorescent product which is read at 460nm in a fluorescent plate reader with excitation at 390nm

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Mechanistically, NAC enhances the synthesis of cysteine and mercapturic acid conjugates, enabling glutathione to neutralise the hepatotoxic metabolite of acetaminophen more rapidly and efficiently, thereby minimising hepatocellular injury (Dodd et al., 2008

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