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Sections were washed thrice in PBS for 5 min and the slides were mounted using Immu-Mount mounting medium (Thermo)

Iron death, characterized by oxidative stress due to intracellular Fe2+ accumulation, leading to large amounts of ROS production, ultimately results in cell death (161) and contributes to various cardiovascular diseases including atherosclerosis, I/R injury, HF, myocardial infarction and adriamycin cardiomyopathy (162)

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