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Results related to GPx activity have also been contradictory, and whether GPx activity decreases (67) or increases with age remains inconclusive
Immune cells use glutathione both as an antioxidant and as a signaling molecule that helps coordinate your bodys defense responses
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Western blot analysis The levels of cellular proteins were quantified using Western blot analysis by utilizing primary antibodies: anti--catenin (1:1000), anti-pGSK3 (Ser9, 1:1000), anti- tGSK3 (1:1000), anti-cyclin D1 (1:1000), anti-p-Akt (1:1000), anti-tAkt (1:1000) (all from Cell Signaling Technology, Danvers, MA, USA), or anti-GAPDH (1:5000