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Biochim Biophys Acta 1985

Then, 0.1% hyaluronidase (H4272, Sigma-Aldrich, Saint Louis, MO, USA) was used to remove cumulus cells

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Results showed that when 20 ppm of the antifolate co-trimoxazole were added, the infection ratio decreased to ~ 10% after 9 days inoculation (for the control, the infection ratio was 100% after 5 days inoculation)