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Need ultrasonic Add each solvent one by one: 0.5% Methylcellulose/0.2% Tween-80 in Saline water Solubility: 2 mg/mL (4.90 mM)

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In Vitro Skin Irritation Test HaCaT keratinocytes were cultured in DMEM containing 10% fetal bovine serum and 1% penicillin/streptomycin and were maintained under 37 C with humidified 5% CO 2

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