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The reaction mechanism is as follows: GSSG + NADPH + H 2 GSH + NADP (catalyzed by GR) It's worth emphasizing that the GSH/GSSG ratio is a crucial indicator of cellular redox status

All P- values are reported in Supplemental Table 1

(3) Ultrasonic extraction in an ice-water bath for 10 min, and leave at -40C overnight

These percentages are almost identical to the percentages for the second-order rate constants of the same mutants with GSSCys as a substrate 14 , indicating that GSH also occupies the 1st glutathione-interaction site of PfGrx for the reduction of roGFP2 WT (S 2 )

The peptide market has seen an influx of new vendors over the past 24 months, many with minimal track records and questionable quality standards

Monks, J