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Studies have shown that the vagus nerve can sense gut microbiota-derived metabolites and modulate central neurotransmitter levels through neuroendocrine signaling (Shaikh et al., 2020)

Preparation of DTNB working solution: Dilute the DTNB stock solution 100-fold with 0.5 mol/L, pH 8.0 Tris-HCl buffer to prepare the DTNB working solution

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