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For example, immortalized neurons and immature primary cortical neurons undergo cell death in response to depletion of the anti-oxidant glutathione, which can be elicited by administration of glutamate at high concentrations

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Interestingly, higher concentrations such as 1 mM and 2 mM did not yield similar protective effects, indicating a dose-dependent response and identifying 0.1 mM as the optimal concentration for reactive oxygen species mitigation [2]

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Ethanol's suppression of GHRH and elevation of somatostatin mean that even though the peptides are present and biologically active, the pituitary's responsiveness is blunted