Post incubation for 10 minutes, the supernatant (saponin lysate) was separated and the parasite pellet (saponin pellet) was washed with PBS
PBMC lysate was utilized in our experimentation to find intracellular cytokine levels
doi: 10.1055/s-2004-820992
doi: 10.4110/in.2014.14.6.277 91 KimH
Martnez-Reyes I, Cardona LR, Kong H, Vasan K, McElroy GS, Werner M, et al
1 Introduction Cynanchum plants have been used to treat a variety of diseases in folk medicine, such as cancer, inflammation, and viral infections ( 21 sterol glycosides, a class of secondary metabolites in Cynanchum plants, are responsible for regulating cellular activities associated with cancer, including cell proliferation, apoptosis, and metastasis ( 21 steroidal glycosides (e.g., dioscin) can have potent anticancer activity by triggering mitochondrial apoptosis, caudatin is structurally unique given its incorporation of an isovalerate side chain and the C-3 glycosylation affords a broader target specificity against Wnt/-catenin and autophagy-lysosomal pathways
