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In vitro studies show that in oxidative conditions, recombinant GSH1 forms homodimers and that reduction of the regulatory disulfide bridge causes dissociation of the homodimer into monomers [99,100]

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Strategies for GSH system activation Identifying strategies that fortify the GSH system in the heart may contribute to developing therapies against cardiac diseases induced by various pathological factors

Stanley Medical Research Institute (USA)

First, a rat was placed in the center chamber (guillotine doors in place) for 5 min

Lyse cells by sonication on ice (~10 times for 10 sec each with 1 min rest between bursts to minimize sample heating)