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After being centrifuged at 12,000 g for 10 min, the total solubilized protein extract was yielded in the supernatant

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Clinical Trial Outcomes with Monotherapy The REDEFINE 1 trial demonstrated that cagrilintide monotherapy at the 4.5 mg maintenance dose produced mean body weight reductions of approximately 10.8% from baseline after 32 weeks of treatment [4]

At 7 d the media was aspirated, and cells were incubated at 4C with 15 ml cold PBS (Gibco, 10010-023) for 10 min

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