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A significant reduction in appetite and food intake was also observed with tirzepatide compared to placebo in people with T2D [45]

Solution selection and preparation The solution used to suspend the crude NPC suspension and to dilute the OptiPrep may be a routine buffered saline such as PBS [4,5], which may be supplemented with 1% BSA [6] or a balanced salt solution such as Hanks Balanced Salt Solution (HBSS) [7-11] or an NPC customized medium such as Geys balanced salt solution [12-15] and this may be prepared as described in the box

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