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Finally, the tissue sections were washed thoroughly with PBS, counterstained with DAPI and imaged under a laser scanning confocal microscope (FV-1000, Olympus, Japan)

Sperm cells are particularly vulnerable to oxidative stress because they have very little cytoplasmthe "cushioning" part of a cellwhere antioxidants are usually stored

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Fragouli E et al (2015) Altered levels of mitochondrial DNA are associated with female age, aneuploidy, and provide an independent measure of embryonic implantation potential

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