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Peptides such as CJC-1295, Ipamorelin, and Sermorelin target GH signaling, while testosterone modulation typically involves different biological pathways

Fluorescence was read (excitation360 nm, emission460 nm) every min for 60-min at 37C using a plate reader (Synergy HT, Biotek)

Starting from day 14, the culture medium was replaced with Hepatocyte Maintenance Medium [Lonza Hepatocyte Culture Medium (HCM) composed of 500 ml 1X HBM Basal Medium and 1X HCM SingleQuots Supplement Pack (both from Lonza Group, Ltd.), supplemented with 20 ng/ml OSM (R&D Systems)]

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