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44 Exploration for the use of extracts from the tropical plant Moringa oleifera is in progress, to determine the antioxidant capacity in the treatment of oxidative stress in sickle cell disease

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For one-step reactions, all components were mixed in 96-well microtitre plates in 100 l volume (PS, F-Bottom, clear, Greiner) and reactions were performed for 30 min in a Spark 20 M (Tecan) plate reader at 30 C

Distinctive chromatin in human sperm packages genes for embryo development

RNA isolation and qRT-PCR Total RNA from the tissues and cells was extracted using the TRIzol method (Takara, Japan)

Zhao M, Liang G, Wu X, Wang S, Zhang P, Su Y, Yin H, Tan Y, Zhang J, Lu Q (2012) Abnormal epigenetic modifications in peripheral blood mononuclear cells from patients with alopecia areata