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Serum was isolated from the blood by centrifuging blood samples at 10,000 g for 5 min and stored at 80 C

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Total biomass at 34 days decreased in reused LMS-1 (ave = 0.4 gm) compared to the Earth soil controls (ave = 0.95 gm, p = 0.014 calculated by one-way ANOVA), but was similar to fresh LMS-1 (ave = 0.22 gm, p = 0.69 calculated as one-way ANOVA) ( Figure 6F )

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