141, no
Compared with mock cells, no apparent differences in concentrations of reduced or oxidized GSH were observed in GSTP1 -transfected cells
In addition to enzyme-to-substrate ratio and time, consider altering buffer conditions, such as increasing or decreasing NaCl concentration or adding Ca 2+ to the buffer (see Note 31 for troubleshooting tips)
Consequently, beyond merely confirming GSH depletion, our method revealed complex metabolic alterations that are rarely quantified directly [74], such as the highly significant intracellular accumulation of gGC and specific shifts in CG, CT, and Tau
Besides a depletion of Gln, GGT from gastric helicobacters also causes degradation of GSH 15,16
sclerotiorum showed a slower rate of Fv/Fm decline than inoculation with strain oah/SsCm1-9 (Fig