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Incubate the culture at 37 C at 250300 rpm until the OD 600 is 0.50.7 (see Note 10)

4 , 17 (2012) Avogadro: an open-source molecular builder and visualization tool

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Lippi G, Montagnana M, Salvagno GL, Guidi GC, Targher G (2008) Prevalence of folic acid and vitamin B 12 deficiencies in patients with thyroid disorders