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To determine the effect of the amino acid treatment on cell viability, HepG2 cells were seeded in 96-well plates at a density of 1 10 5 cells/well and cultured for 24 h

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As a negative control, 5 mM sodium azide was added to a separate control well 5 min before LPFC analysis to irreversibly inhibit cytochrome C oxidase and establish a baseline for low ATP levels

J., & Shenoy, V